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ISSCR - Viacyte poster

Discussion in 'Research' started by Deal, Jun 29, 2016.

  1. Deal

    Deal Approved members

    Joined:
    Nov 2, 2009
    Messages:
    557
    Did anyone attend?

    http://viacyte.com/press-releases/viacyte-to-present-at-isscr-2016-annual-meeting/

    The ISSCR is a conference on Stem Cell research held last week. There were 10 or more posters that contained the word diabetes indicating that lots of stem cell research is ongoing within the field. The language in the posters is highly technical/scientific so I am unable to summarize other than to say work is in progress.

    The Viacyte research gives me hope and one researcher from that team participated in a session.

    All Abstracts (600+ pages): http://www.isscr.org/docs/default-source/2016-books/isscr-2016-poster-abstract-book.pdf

    Viacyte abstract:

    T2190
    IN VITRO GENERATION OF INSULINPRODUCING
    CELLS WITH INSULIN
    SECRETION KINETICS AND MITOCHONDRIAL
    RESPIRATION SIMILAR TO ADULT HUMAN
    ISLETS
    Rezania, Alireza1, Arora, Payal1, Rieck, Sebastian1,
    Ng, Bruce1, Asadi, Ali2, Quiskamp, Nina2 and Kieffer,
    Tim3, 1Janssen, Raritan, NJ, U.S., 2University of British
    Columbia, Vancouver, BC, Canada, 3University of
    British Columbia, Vancouver, BC, Canada
    We recently reported on the differentiation of pluripotent
    human stem cells into single hormonal insulin+ NKX6.1+
    MAFA+ cells (stage 7 cells) that demonstrated glucose
    responsiveness in a static glucose challenge assay. Further
    evaluation using a more sensitive dynamic perifusion
    assay revealed sluggish insulin release kinetics in response
    to glucose challenge and marginal increase in insulin release
    after exposure to a GLP-1 mimetic peptide. Moreover,
    the single hormonal insulin+ cells did not show the
    typical calcium flux seen with adult human beta cells indicating
    clear functional deficiencies as compared to mature
    beta cells. Using a combination of RNA Seq analysis
    of the cells, empirical testing of various media compositions
    coupled with targeted small molecule screening, we
    generated insulin+ cells from human embryonic stem cells
    (hESCs) with insulin secretion kinetics and mitochondrial
    respiration similar to adult human islets. Newly generated
    insulin+ cells acquired some of the features attributed
    to mature beta cells, such as expression of UCN3, MAFA,
    GLP-1r, and GLUT1. Notably, these insulin+ cells survived
    within macroencapsulation devices following transplant
    in the subcutaneous space of immune compromised mice
    and yielded significantly faster production of circulating
    human C-peptide compared to previous reports, with
    significant glucose responsiveness as early as four weeks
    post-implant. These results show the feasibility of generating
    insulin-producing cells from hESCs in vitro with
    characteristics of mature human beta cells.
     
  2. samson

    samson Approved members

    Joined:
    May 11, 2016
    Messages:
    119
    This is good progress but I'm holding out hope for Martin Fussenegger's approach
    Work with hESCs is always prone to tumor formation, so that makes me wary.
    Also, I thought ViaCyte's work was already in clinical trials?
    Are these cells updates on what they're already testing in clinical trials?
     

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